Abstract: OBJECTIVE To observe the effects of total flavonoids from Patrinia on oxidative stress and energy metabolism in rats with focal cerebral ischemia reperfusion injury. METHODS Ninety-eight Wistar rats were randomly divided into sham operation group, model group, nimodipine group(20 mg·kg^-1), Naoluotong group(500 mg·kg^-1), high-dose, medium-dose, low-dose (200, 100, 50 mg·kg^-1) Patrinia total flavonoids groups. Stomach irrigation volume was 20 mL·kg^-1, once a day for 7 d. Middle cerebral artery occlusion models of rats were made with thread blocking method. Drugs were given to the rats 7 d before preparation of the models, ig for consecutive 7 d. After 22 hours of reperfusion, the neurological function score was measured, and the levels of ATPase, superoxide dismutase(SOD) and malondialdehyde(MDA) in brain tissue were measured. The histopathological changes of hippocampus and cortex were observed by HE staining. RESULTS Compared with the sham operation group, the neurological deficit score in the model group increased significantly, Na⁺-K⁺-ATP and Mg²⁺-ATP enzyme activities decreased significantly, MDA content in the brain homogenate increased significantly, and SOD content decreased significantly(P<0.01). Compared with the model group, total flavones of Patrinia could decrease the scores of neurological deficit, increase the levels of SOD and ATP in brain tissue, decrease MDA levels(P<0.01 or P<0.05), and improve the pathological damage of hippocampus and cortex in brain tissue. CONCLUSION Total flavones from Patrinia have better protective effect on focal cerebral ischemia-reperfusion injury. It can improve the level of oxidative stress and could improve the pathological changes of cerebral cortex and hippocampus. The mechanism of action may be related to improving energy metabolism of brain tissue and reducing free radical injury.