Abstract: Fluorous solid-phase extraction(FSPE) is a solid-phase extraction technology based on fluorine-fluorine interactions. Retaining on the perfluoroalkyl-functionalized stationary phase, and then eluted with a fluorophilic reagent, the perfluoroalkyl-containing target compound is separated and purified. In recent years, FSPE has been widely used in the enrichment and purification of peptides. However, because peptides normally do not contain highly fluorinated functional groups, attaching such highly fluorinated tags to peptide molecules has become the key to the application of FSPE to peptide analysis. This review summarized and elaborated the strategies and types of introduction of fluorinated affinity tags into peptides, their applications and related technologies in peptide synthesis, purification and enrichment.