Abstract: OBJECTIVE To establish a rapid qualitative and quantitative analysis model of chondroitin sulfate eye drops by Raman spectroscopy, and to improve the prediction ability of the model. METHODS The standard Raman spectra of chondroitin sulfate were used as reference spectra, and the interference spectra, spectral range and pretreatment methods were set up in RFDI software to establish the qualitative analysis model. Twenty-three batches of representative samples were selected as the reference spectra, and the quantitative analysis model was established by cross-examination based on the determination data of chondroitin sulfate content. RESULTS The established qualitative model was used to test chondroitin sulfate eye drops. Thirty nine batches of single samples passed and 4 batches of compound samples failed. The cross validation of the quantitative model results showed that the root mean square error was 1.43 mg·mL^-1, the correlation coefficient was 0.985 4, and the concentration range was 26.19-35.85 mg·mL^-1. CONCLUSION Raman spectroscopy can be used to establish a qualitative model of chondroitin sulfate eye drops with high accuracy and fast analysis speed. Based on reliable laboratory content analysis data, a quantitative model of chondroitin sulfate eye drops can be established to rapidly analyze the content of chondroitin sulfate, which can used in rapid identification and determination.