高效液相色谱法-荧光检测器测定复方氨基酸注射液中铝元素含量

Determination of Aluminum in Compound Aminoacid Injection by HPLC-Fluorescence Detector

摘要: 目的建立复方氨基酸注射液中铝元素的高效液相色谱测定方法。方法样品经酸水解后，游离态的铝元素与流动相中的8-羟基喹啉结合，形成荧光配合物，高效液相色谱-荧光检测器进行测定，采用标准曲线法计算铝元素的含量。结果铝元素在5~200 μg·L^-1线性关系良好，精密度、重复性的RSD均<3%，加样回收率在93.15%~110.07%。结论方法易操作，灵敏度高，准确度好，为注射液中铝元素的含量测定提供有效的方法。

Abstract: OBJECTIVE To establish a HPLC method for the determination of aluminum in compound aminoacid injections. METHODS After acid hydrolysis, the free aluminum was combined with 8-hydroxyquinoline in the mobile phase to form a fluorescent complex. HPLC and fluorescent detector was used to determine the content of aluminum by standard curve method. RESULTS The linear relationship of aluminum was good in the range of 5-200 μg·L^-1. The precision and repeatability of RSD were <3%, and the recovery rates were 93.15%-110.07%. CONCLUSION The method is easy to operate, high sensitivity and good accuracy. It provides an effective method for the determination of aluminum in the injection.