HPLC测定酮缬氨酸钙含量的方法优化

Improvement of Content Determination for α-Ketovaine Calcium by HPLC

摘要: 目的优化HPLC测定酮缬氨酸钙含量的方法。方法 HPLC采用CAPCELL PAK C₁₈ MGⅡ柱（250 mmx4.6 mm，5 μm），流动相为高氯酸钠溶液（pH 2.5）-乙腈（96∶4），柱温为35℃，检测波长205 nm，流速1.0 mL·min^-1。结果酮缬氨酸钙在0.025~0.185 mg·mL^-1内有良好的线性关系（r=1.000 0），平均回收率（n=9）为100.0%。结论该方法简便、快速、准确，灵敏度高，重复性好，可用于酮缬氨酸钙的含量测定。

Abstract: OBJECTIVE To improve an HPLC method for the content determination of α-ketovaine calcium. METHODS The HPLC method was performed on CAPCELL PAK C₁₈ MGⅡ column(250 mm×4.6 mm, 5 μm) with mobile phase of sodium perchlorate buffer(pH 2.5)-acetonitrile(96:4). Column temperature was 35℃, detective wavelength was 205 nm, and the flow rate was 1.0 mL·min^-1. RESULTS The linear range of α-ketovaine calcium was 0.025-0.185 mg·mL^-1 (r=1.000 0), the average recovery(n=9) was 100.0%. CONCLUSION The method is simple, rapid and accurate with good repeatability, which can be used for the determination of α-ketovaine calcium.