Abstract: OBJECTIVE To explore the effects of novel compound F-2 on proliferation, migration and apoptosis of human non-small cell lung cancer NCI-H520 cells. METHODS The proliferation of lung cancer cells was detected by CCK-8 test. The migration of NCI-H520 cells was detected by wound scratch test. The effect of compound stimulation on cell morphology was observed by inverted microscope. The effect of compounds on apoptosis was detected by flow cytometry. The effects of compounds on cell migration and apoptosis-related protein expression were detected by Western blotting. RESULTS The compounds significantly inhibited the proliferation of NCI-H520 cells and was dependent on concentration and time. The Wound scratch test showed that the compound could significantly inhibit the migration of tumor cells. Western blotting results showed that the compound could up-regulate the expression of E-cadherin and down-regulate the expression of N-cadherin in NCI-H520 cells. Annexin-V/PI double staining showed that the compound could induce apoptosis of NCI-H520 cells, and the apoptotic rate increased with the increase of drug concentration, and the expression ratio of BAX/Bcl-2 protein increased. CONCLUSION Compounds can significantly inhibit the proliferation, migration and induce apoptosis of lung cancer cells, which may be through blocking the epithelial-mesenchymal transition process of tumor cells and regulating the expression of related genes during apoptosis.