Abstract: OBJECTIVE To establish a quantitative analysis of multi-components by single-marker(QAMS) method for simultaneous determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, polydatin, emodin, physcion, diosgenin, oleanolic acid, ursolic acid, euscaphic acid and pomolic acid in Yishen Yangyuan granules. METHODS HPLC analysis was performed on an Spursil C₁₈(4.6 mm×250 mm, 5 μm) with a mobile phase consisted of methanol-0.1% formic acid solution in gradient elution manner at a flow rate of 1.0 mL·min^-1. The column temperature was maintained at 30℃. The detection wavelength were 280 nm for 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, polydatin, emodin, physcion, and 210 nm for diosgenin, oleanolic acid, ursolic acid, euscaphic acid, pomolic acid. Diosgenin was used as the internal reference substance, its relative correction factor with other components was established and its content was calculated, at the same time, the method was evaluated by comparison of the quantitative results between external standard method and QAMS method. RESULTS 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside, polydatin, emodin, physcion, diosgenin, oleanolic acid, ursolic acid, euscaphic acid and pomolic acid had good relations within the range of 23.89-477.80, 8.67-173.40, 4.26-85.20, 2.99-59.80, 9.86-197.20, 0.69-13.80, 2.17-43.40, 1.28-25.60, 1.66-33.20 μg·mL^-1(r=0.999 4-0.999 9), The average recovery were 99.45%, 98.49%, 97.94%, 98.91%, 100.03%, 96.90%, 99.02%, 97.86% and 98.35%, RSDs were 1.01%, 0.87%, 1.37%, 1.19%, 0.70%, 0.98%, 1.03%, 0.82% and 1.53%(n=9), respectively. There was no significant difference between the calculated and measured values of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, polydatin, emodin, physcion, diosgenin, oleanolic acid, ursolic acid, euscaphic acid and pomolic acid according to T-test analysis. CONCLUSION The QAMS method can simultaneously determine the content of nine components in Yishen Yangyuan granules, the method is simple and accurate.