Abstract: OBJECTIVE To establish the TLC identification and HPLC quantitative analysis method for Artemisa sacrorum Ledeb. METHODS TLC and HPLC were used for qualitative and quantitative analysis of Artemisa sacrorum Ledeb collected from five different regions in Tongliao, Inner Mongolia. TLC conditions were as follow:The reference substances were 8-hydroxy-6,7-dimethoxycoumarin and 7-hydroxy-6-methoxycoumarin, the adsorbent with silica gel G, the developing agent with dichloromethane-acetone(7:1) and the chromogenic reagent with ultraviolet lamp; The reference substance was 6,8-dimethoxycoumarin-7-O-β-D-glucoside, the adsorbent with silica gel G, the developing agent with dichloromethane-methanol(5:1) and the chromogenic reagent with ultraviolet lamp. HPLC conditions were as follow:The separation was performed on Topsil C₁₈(250 mm×4.6 mm, 5 μm); The mobile phase was comprised of water(A)-acetonitrile(B) with a gradient elution. The detection wavelength was 263 nm with column temperature at 30℃. RESULTS The spots in TLC of reference substances(8-hydroxy-6,7-dimethoxycoumarin, 7-hydroxy-6-methoxycoumarin and 6,8-dimethoxycoumarin-7-O-β-D-glucoside) and different samples had the same Rf value, with good repeatability and easy to be identified. Under the HPLC conditions adopted in this study, all calibration curves compound 1-7 exhibited good linearity(r ≥ 0.999 3). The recoveries of the method were 98.5, 95.0, 97.0, 98.3, 96.0, 98.9, 99.1, respectively. RSD were <2.0%. CONCLUSION A simple, stable and reliable method for qualitative and quantitative analysis method of Artemisa sacrorum Ledeb, which provides a basis for comprehensive evaluation of the quality of Artemisa sacrorum Ledeb.