Abstract: OBJECTIVE To investigate the action mechanism by detecting the ginsenoside content changes in panax genseng, red ginseng, white ginseng with different proportions of trogopterus dung. METHODS The separation was performed on Agilent Athena C₁₈(4.6 mm×250 mm, 5 μm) column and the mobile phase consisted of acetonitrile(A) and H₃PO₄(B, pH6.8) water solution in gradient elution at flow rate of 1.0 mL·min^-1, the column temperature was 25℃, the detection wavelength was set at 203 nm. RESULTS The linear ranges were 0.036 2-1.16 μg(r=0.998 5) for Rg₁, 0.037 1-1.19 μg(r=0.999 0) for Re, 0.030 3-0.97 μg(r=0.991 4) for Rf, 0.035 0-1.12 μg(r=0.993 9) for Rb₁, 0.030 6-0.98 μg(r=0.997 3) for Rc, 0.030 3-0.97 μg (r=0.999 1) for Rb₂, 0.031 2-1.00 μg(r=0.999 1) for Rd. The sample recovery rate RSD <3%, the stability, repeatability and precision of the method were good(RSD <3%). CONCLUSION The contents of seven ginsenoside decreased significantly when panax ginseng, red ginseng, white ginseng combined with trogopterus dung, and when the ratio was 1:4, Rb₁ and Rb₂ decreased the maximum, highest fall achieve 90%. The content of Rg₁ reduced the minimum, but also fell more than 70%.