Abstract: OBJECTIVE To improve the quality standards of Thalictri Radix et Rhizoma, and provide the basis for its further development. METHODS On the basis of market research, the current commodity situation had been grasped by the collection of samples, consulting of specimens in the plant specimen museum and comparative study. The TLC method was used for qualitative identification of Thalictri Radix et Rhizoma. The content of berberine hydrochloride in Thalictri Radix et Rhizoma was determined by HPLC. RESULTS Thalictri Radix et Rhizoma in Gansu province were mainly derived from Thalictrum baicalense Turcz. The TLC spots were clear and well separated, and the linear range of berberine hydrochloride was 5.0-160.0 μg·mL^-1. RSDs of precision, repeatability and stability tests were <1.8%. The recoveries were 101.99%(RSD=2.53%, n=6). CONCLUSION This method established in this study is simple and accurate, which is suitable for the quality control of Thalictri Radix et Rhizoma.