Abstract: OBJECTIVE To establish HPLC characteristic chromatogram and simultaneous determination of six components for Yinhuang capsules. METHODS The HPLC analysis was carried out on a column of Agilent ZORBAX SB-C₁₈ (250 mm×4.6 mm, 5 μm) with the temperature of 30℃. The mobile phase was consisted of acetonitrile-0.4% acetic acid with gradient at the flow rate of 1.0 mL·min^-1, the detection wavelength was 327 nm and the injection volume was 10 μL. Characteristic chromatograms of 20 batches of Yinhuang capsules were studied and evaluated by "Similarity Evaluation System for Chromatographic Fingerprint of TCM Software (2012. 130723 Version)", and the characteristic peaks in the fingerprint were identified by standard substances. RESULTS The HPLC characteristic chromatograms for 20 batches of the samples were established, and six characteristic peaks were indentified. And the method of simultaneous determination for six components was established. The linear ranges of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3-5-O-dicaffeoylquinic, baicalin and baicalein were 0.638 7-6.387 μg·mL^-1(r=0.999 3), 2.893-28.93 μg·mL^-1(r=0.999 8), 0.664 1-6.641 μg·mL^-1(r=0.999 7), 0.743 1-7.431 μg·mL^-1(r=0.999 6), 16.19-161.9 μg·mL^-1(r=0.999 7) and 2.143-21.43 μg·mL^-1(r=0.999 7), respectively. The average recoveries(n=6) of six components were 100.19% (RSD=1.76%), 101.14% (RSD=1.29%), 98.86% (RSD=1.20%), 98.05% (RSD=0.99%), 99.38% (RSD=1.92%) and 99.70% (RSD=1.92%), respectively. The contents of six components in 20 batches of Yinhuang capsules were 0.637 9-4.100, 3.069-15.28, 0.960 4-4.906, 0.625 9-4.440, 82.69-118.2 and 2.118-14.76 mg·g^-1, respectively. CONCLUSION The proposed specific HPLC characteristic chromatograms and quantitation method of six components can provide scientific basis for quality control of the Yinhuang capsules.