Abstract: OBJECTIVE To explore the mechanism and principle of Morindae Officinalis Radix in the treatment of osteoporosis based on network pharmacology. METHODS The active ingredients and related target proteins of Morindae Officinalis Radix were extracted from TCMSP. The relationship network between active components and target proteins of Morindae Officinalis Radix was constructed by using the visualization software Cytoscape 3.5.1 for topological analysis. And the STRING 10.5 was used to construct protein-protein interaction network and analyze data. The enrichment of biological signaling pathway function was analyzed by using David v6.8. RESULTS The core compounds of Morindae Officinalis Radix were beta-sitosterol, 2-hydroxy-1,5-dimethoxy-6-(methoxymethyl)-9,10-anthraquinone, alizarin-2-methylether, and other key targets were prostaglandin G/H synthase 2, heat shock protein 90, progesterone receptor, etc. The active components of Morindae Officinalis Radix mainly acted on the key proteins, such as ESR1, JUN,CASP3, PGR, CASP8, etc. KEGG results showed that Morindae Officinalis Radix acted on Estrogen signaling pathway, PI3K-Akt signaling pathway and other signaling pathways. CONCLUSION Morindae Officinalis Radix plays an anti-osteoporosis role mainly through a variety of pathways and signaling pathways acting on a variety of targets. At the same time, it is predicted that the anti-osteoporosis effect of Morindae Officinalis Radix may be related to anthraquinone compounds, cGMP-PKG signaling pathways and target proteins such as ESR1, JUN, etc.