Abstract: OBJECTIVE To establish a method based on molecular biology technology to identify animal-derived components in ossotide preparations, and the species of bone raw materials used by some domestic ossotide preparations enterprises were identified. METHODS The intermediate in the process of ossotide preparations was taken as the sample, and nucleic acid was extracted by optimized CTAB method. The animal-derived components were identified by PCR amplification and agarose gel electrophoresis. RESULTS The samples from 9 enterprises were identified by the method successfully established for ossotide extraction intermediate, and only pig source components were detected, no cattle or sheep source components were detected in all samples. CONCLUSION All the 9 enterprises use pig bone as raw material for ossotide preparations, which is in line with relevant pharmaceutical standards.