Abstract: OBJECTIVE To explore the effect of sublingual immunotherapy(SLIT) with standardized dermatophagoides farinae drops on serum sIgG4, IL-10 and IL-33 levels and evaluate the clinical efficacy of SLIT for the treatment of allergic rhinitis(AR) in children. METHODS Seventy children patients with dust-mite AR collected from Ningbo Medical Center of Lihuili Hospital between February and August 2016 were randomly assigned to SLIT group(SLIT combined with pharmacotherapy, n=35) and control group(only pharmacotherapy, n=35). Serum levels of sIgG4, IL-10 and IL-33 were measured by ELISA before and after SLIT for 6 months, 1 year and 2 years, total nasal symptoms score(TNSS) and total medication score(TMS) were evaluated. RESULTS TNSS and TMS scores of SLIT group was significantly lower than the control group after 2 years' treatment(P<0.01). Compared with before treatment, the levels of sIgG4 and IL-10 increased significantly and IL-33 decreased significantly in children with AR after 2 years' SLIT(P<0.05). The level of sIgG4, IL-10 and IL-33 in control group did not differ significantly before and after 2 years' treatment. Meanwhile, a significant difference was found in the expression of sIgG, IL-10 and IL-33 between two groups at the end of 2 years' treatment(P<0.05). CONCLUSION SLIT with dermatophagoides farinae drops is efficient treatment for children with HDM-induced AR. The change of serum sIgG4, IL-10 and IL-33 level may be used as an indicator to assess the clinical efficacy of SLIT.