Abstract: OBJECTIVE To establish an analysis method of QAMS for simultaneous determination for three active components in Dongju Liyan mixture including chlorogenic acid, cynaroside and 3,5-O-dicaffeoylquinic acid. METHODS HPLC method was used and the analysis was performed on Agilent TC-C₁₈(2)(4.6 mm×250 mm, 5 μm) column. The mobile phase was acetonitrile-0.1% H₃PO₄ solution with gradient elution. The flow rate was 1.0 mL·min^-1. The column temperature was 30℃ and the detection wavelength was 334 nm. The relative correction factors(f_s∕i) of chlorogenic acid as internal reference to cynaroside and 3,5-O-dicaffeoylquinic acid were calculated. The content of chlorogenic acid was determined by external standard method and two other components were calculated by f_s∕iin Dongju Liyan mixture. The results between external standard method and QAMS method were compared with t-test to verify the accuracy of QAMS method. RESULTS The results showed that the f_s∕i had good system applicability and reproducibility, and there was no significant difference between external standard method and QAMS method. CONCLUSION The QAMS method established can be used for quantitative analysis and quality evaluation of Dongju Liyan mixture.