Abstract: OBJECTIVE To explore the quality inspection method of flower of Trollius chinensis. METHODS The quality of the flower of Trollius chinensis was systematically investigated by means of apparent inspection, TLC, IR and HPLC. RESULTS Examined samples of 10 batches of flower of Trollius chinensis from different producing areas. Its apparent inspection description was characterized by strong features, and the spots of TLC were clear and the separation effect was good. The established HPLC standard fingerprint method of flower of Trollius chinensis had good precision, repeatability and stability, and the IR fingerprint method could be quickly analysed. CONCLUSION A variety of methods were used to analyze the quality of the flower of Trollius chinensis, and established a relatively complete inspection method system, which provides a certain method support for the rapid identification of flower of Trollius chinensis, which can be used for quality analysis and comprehensive evaluation of flower of Trollius chinensis.