Abstract: OBJECTIVE To analyze the effects of spleen polypeptide injection adjuvant therapy on T lymphocyte and immunologic factors in patients with colorectal carcinoma, to provide reference for clinical treatment of patients with colorectal carcinoma. METHODS A total of 88 advanced colorectal carcinoma patients with unresectable liver metastasis who were dignoised and inclusion criteria in the First Affiliated Hospital of Nanjing Medical University were selected from March 2016 to April 2018, and they were divided into observation group(n=44) and control group(n=44) according to the random number table method. Patients in the control group were treated with conventional chemotherapy, and the observation group was treated with spleen polypeptide injection on the basis of the control group. The clinical effect, the levels of T lymphocyte and immunologic factors were compared between the two groups. RESULTS The total effective rate of clinical treatment in observation group was obviously higher than that of control group (P<0.05). Before treatment, there were no significantly differences in the level of T lymphocyte and immunologic factors between the two groups. After treatment, the level of CD₃⁺, CD₄⁺, CD₄⁺/CD₈⁺ and natural killer in the two groups were obviously higher than those of before treatment(P<0.05), and the observation group was obviously higher than the control group(P<0.05). The level of CD₈⁺, soluble interleukin-2 receptor and interleukin-8 in the two groups were obviously lower than those of before treatment(P<0.05), and the observation group was obviously lower than the control group(P<0.05). CONCLUSION The spleen polypeptide injection adjuvant therapy can improve the clinical effects of patients with colorectal carcinoma, and improve the levels of T lymphocyte and immunologic factors.