Abstract: OBJECTIVE To establish the method of HPLC fingerprint analysis of coumarins for the quality control of Peucedani Radix. METHODS The HPLC fingerprint were obtained on an Ecosil-C₁₈(4.6 mm×250 mm, 5 μm) with the isocratic elution solvent system composed of methanol and water(68:32). The analysis time was 50 min, the flow rate was 1.0 mL·min^-1, the column temperature was maintained at 40℃, and the detection wavelength was set at 323 nm. LC-MS was used to analyze the composition of adulterants of Peucedani Radix, and to identified the characteristic peaks. Similarity of 18 batches of Peucedani Radix samples and 4 batches of adulterants were evaluated by chromatographic fingerprint similarity evaluation software. RESULTS The HPLC fingerprint of Peucedani Radix was established based on the reference of praeruptorin A and praeruptorin B. The detected peaks preliminarily belonged to qianhucoumarin D, qianhucoumarin E, peucedanumarin II, praeruptorin A, peucedanumarin I, praeruptorin B, praeruptorin E and its isomer by qualitative analysis of LC-MS combined with reference comparison. In addition, the similarity was 0.9, which could distinguish Peucedani Radix and its confusion well. CONCLUSION The method is simple and reproducible, and can provide an effective identification method for the quality evaluation of Peucedani Radix.