Abstract: OBJECTIVE To study the effects of Ginkgo Folium extract combined with prednisone on Ghrelin-Obestatin signaling pathway in mice with chronic obstructive pulmonary disease(COPD). METHODS Forty healthy SPF mice were randomly divided into 5 groups, the blank group was not treated, and the other 4 groups of mice were induced with COPD model. On the 8th day of modeling, the three groups were treated with Ginkgo Folium extract(0.4 mL·kg^-1·d^-1), prednisone(10 mg·kg^-1·d^-1) and combination therapy(0.4 mL·kg^-1 Ginkgo Folium extract+10 mg·kg^-1 prednisone), while the blank group and model group were given normal saline. The mice were examined for lung function PIF, PEF and MV using the Buxco system and then the mice were sacrificed after 28 d of continuous administration. Blood samples and lung tissue samples were taken. HE staining was used to investigate the pathological changes of lung tissue; RT-PCR was used to detect the expression of Ghrelin, GHSR and GPR39 mRNA in lung tissue; Western blotting was used to detect the content of Ghrelin, GHSR, Obestatin and GPR39 in lung tissue; serum levels of TNF-α, IL-6 and IL-8 were measured by ELISA. RESULTS Compared with model group, different groups of administration alleviated the pathological damage of lung tissue in COPD mice, and the effect of combination group was the best. The PIF, PEF and MV of pulmonary function in combination group were significantly higher than model group(P<0.05); the mRNA and protein expression of Ghrelin, GHSR and GPR39 in combination group was significantly higher than that in model group(P<0.05), and the expression of Obestatin protein was significantly higher than that in model group(P<0.05); the levels of serum TNF-α, IL-6 and IL-8 in combination group were significantly lower than those in model group(P<0.05), and the effect was better than single drug groups. CONCLUSION Ginkgo Folium extract combined with prednisone can affect the Ghrelin-Obestatin signaling pathway, significantly reduce inflammation and improve lung function and it is better than single drug.