Abstract: Multidrug resistance(MDR) is a major obstacle to successful cancer treatment. Nanocarrier-mediated co-delivery is an effective strategy for overcoming MDR, which possess great advantages for tumor targeting, controlled drug release, and identical drug PK profiles. In this review, a summary of various design strategy of nanocarrier-mediated co-delivery was presented, and highlighted the recent advances in nanocarrier-mediated co-delivery against cancer drug resistance, including co-encapsulation of drugs with ratiometric control over drug loading, temporal sequencing on drug release, and tumor microenvironment. These emerging strategies promise novel and better tailored combinatorial regimens for clinical cancer treatment.