Abstract: Lopinavir, ritonavir and ribavirin are recommended in Diagnostic and Treatment Scheme for COVID-19(Trial Version 5). The research progress of clinical drug analysis of lopinavir, ritonavir and ribavirin in recent years is summarized. The focus is on a variety of methods for the determination of drugs in plasma, including HPLC, LC-MS, MS, spectrophotometry, capillary electrophoresis, immunoassay, etc., which provide a reference for the development of therapeutic drug monitoring.