Abstract: OBJECTIVE To study the blood compounds in DulingWan and to further explore its pharmacodynamic basis. METHODS The drug-contaning serum and blank serum were prepared by orally administration of migraine mice. Rapid identification of compounds absorbed into blood was carried out by HPLC, according to retention time and reference substances comparison, then preliminary determination of constituents absorbed into blood of mice after oral administration of Duliang Wan, Stewed Angelica dahurica and Ligusticum chuanxiong was investigated. RESULTS After the administration of Duliang Wan, totally 22 compounds absorbed into blood were detected in serum, 18 of them were prototype compounds and 4 components were metabolites of the prototype components. And 12 prototype components were confirmed by reference substance. CONCLUSION These 22 compounds absorbed into blood may be substances that act directly in vivo of Duliang Wan, and it is helpful to clarify mechanism of this herb, quality control standard and dosage form reform.