Abstract: OBJECTIVE To establish the fingerprint of Forsythiae Fructus and conduct a systemic and comprehensive quality evaluation using pattern recognition and quantitative analysis of multi-components by single marker(QAMS). METHODS Ten batches of Forsythiae Fructus were determined by HPLC and a common mode of fingerprint was established. The similarities between fingerprints of 10 batches samples and common mode were evaluated. The further qualitative analysis of the drug was carried out by similarity evaluation, cluster analysis(CA), principal component analysis(PCA). QAMS was performed. Forsythin was selected as internal reference and the relative correction factors(RCF) of forsythiaside A, (+)pinoresinol-β-D-glucoside and phillygenin to it were calculated. At the same time, the content of 4 components in 10 batches of forsythia was measured by external standard method, and the difference between the calculated value and the measured value was compared to verify the accuracy of QAMS. RESULTS There were 13 common peaks in fingerprints of 10 batches of Forsythiae Fructus, four of which were identified. The similarities of fingerprints were >0.98. The batches of the drug were divided into 3 categories by CA and PCA. The established RCF had a good reproducibility. The correlation coefficients between the content values calculated using the correction factors and the measured values were all >0.99, and the relative errors were all <3%. CONCLUSION The developed method is accurate, simple and feasible. The quality of Forsythiae Fructus could be evaluated qualitatively and quantitatively using a fingerprint combined with pattern recognition technique and QAMS technique. Moreover, it will also lay a solid scientific basis for the further quality control of Forsythiae Fructus.