Abstract: OBJECTIVE To explore the mechanism of Atractylodis Rhizoma-Scrophulariae Radix drug pair against type 2 diabetes mellitus using network pharmacology. METHODS The chemical components and targets of Atractylodis Rhizoma-Scrophulariae Radix were searched from TCMSP database and Cytoscape 3.6.1 was used to build a network between components and targets. Secondly, "type 2 diabetes mellitus" targets were searched form TTD, and targets retrieved were used to build a PPI network based on the analysis by STRING database. To obtain type 2 diabetes mellitus targets of the active components, the PPI network was fused with the component-target network. Finally, the DAVID database was used to perform KEGG pathway enrichment analysis in order to explore the mechanism of Atractylodis Rhizoma-Scrophulariae Radix drug pair against type 2 diabetes mellitus. RESULTS The 27 kinds of effective compounds were obtained, 10 target proteins and 6 enrichment pathways were selected. Key targets included PTGS2, DPP4, PTGS1, NR3C2, etc. Regulation included regulation of lipolysis in adipocytes, PPAR signaling pathway, insulin resistance, AMPK signaling pathway, etc. CONCLUSION The active components of Atractylodis Rhizoma-Scrophulariae Radix act on insulin receptor in the treatment of type 2 diabetes mainly through PTGS2, DPP4, PTGS1, NR3C2, etc. This study provide a new foundation for further study on the mechanism of Atractylodis Rhizoma-Scrophulariae Radix composition.