Abstract: OBJECTIVE To observe the effect of rose, astragalus and angelica decoction on the changes of IL-2 and secreted protein acidic and rich in cysteine(Sprac) expression during perimenopause period in rats. METHODS Peri-menopausal syndrome models were established by surgical removal of the bilateral ovaries of female Wistar rats. After successful modeling by detecting the content of estradiol(E2) by ELISA on the 7th postoperative day, the rats were randomly divided into model group, rose, astragalus and angelica decoction low, medium and high dose groups(1.4, 2.8, 5.6 g·kg^-1) and estradiol valerate tablet group(0.09 mg·kg^-1) in the second week after successful modeling, each for 10 rats. Another 10 female Wistar rats were picked as the normal group. 24 h after the last administration, abdominal aortic blood was took to measure the indexes of the uterus, thymus and spleen was checked by weighing method, HE staining to observe uterus tissue morphology, the concentration of E2 and IL-2 in serum was measured by ELISA, Sparc gene expression of uterus tissue was checked by real-time fluorescence quantitative PCR, estradiol receptor α(ERα) protein expression of uterus tissue was determined by immunohistochemistry. RESULTS Compared with the model group, the weight of rats in rose, astragalus and angelica decoction middle, high dose group and estradiol valerate tablet group was significantly lower, the index of uterus, thymus and spleen were significantly higher, the gene expression of Sparc was raised and the protein of ERα were also significantly higher(P<0.05); serum E2 and IL-2 in rose, astragalus and angelica decoction treatment group and estradiol valerate tablet group were significantly higher(P<0.05). The glands appeared and monolayer columnar epithelial cells arranged neatly in high dose group of astragalus and angelica decoction treatment group and estradiol valerate tablet group by HE staining. CONCLUSION Rose, astragalus and angelica decoction can regulate beneficially the unbalanced reproductive endocrine-immune net-work. It can provide experimental basis for clinical application and new perimenopause drugs development.