Abstract: Peroxisome proliferator activated receptors(PPARs) are ligand-activated receptors in the nuclear hormone receptor family, including three subtypes:PPAR_α, PPAR_β/δ and PPAR_γ. PPAR_γ has many biological functions, such as enhancing insulin sensitivity, regulating sugar balance, adipose differentiation and formation. The study of PPAR_γ binding to ligand by fluorescent probe method has great significance for studying the mechanism of PPAR_γ agonist and screening PPAR_γ agonists. This review summarizes the novel fluorescent probe method to explore the binding ability of PPAR_γ to ligands and the screening of PPAR_γ agonists.