Abstract: OBJECTIVE To establish the HPLC fingerprint of Xingren Zhike mixture and determine the contents of 7 chemical compositions. METHODS HPLC-DAD analysis was performed on Agilent ZORBAX Eclipse Plus C₁₈(250 mm×4.6 mm, 5 μm) with the column temperature of 30℃ and the injection volumn was 10 μL. The mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) with gradient elution at the flow rate of 1.0 mL·min^-1. The detection wavelength was 210 nm for amygdalin and tenuifolin; 237 nm for liquiritin; 251 nm for ammonium glycyrrhizate; 284 nm for hesperidin, naringin and neohesperidin. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate the similarity and the cluster analysis was conducted for the results of content determination by SPSS 22.0 statistical software. RESULTS There were 20 common peaks in the HPLC fingerprints of 15 batches of samples with the similarities > 0.90. The linear ranges were 31.97-1 278 μg·mL^-1 for amygdalin, 26.40-1 056 μg·mL^-1 for tenuifolin, 40.38-1 615 μg·mL^-1 for liquiritin, 2.252-90.10 μg·mL^-1 for naringin, 7.698-307.9 μg·mL^-1 for hesperidin, 3.742-149.7 μg·mL^-1 for ammonium glycyrrhizate, 3.032-121.3 μg·mL^-1 for neohesperidin. The limits of quantitation were ≤ 1.901 μg·mL^-1, with r ≥ 0.999 1. RSDs of precision, reproducibility and stability tests (48 h) were ≤ 2.0% (n=6 or n=7). Average recoveries were 99.1%, 96.2%, 101.1%, 96.9%, 97.4%, 98.1%, 99.2%(n=9), respectively. The samples of 15 batches of Xingren Zhike mixture could be divided into 3 categories. CONCLUSION The method is simple and accurate with good repeatability, which can be used for the identification and evaluation of Xingren Zhike mixture, and provides important scientific basis for the quality control of Xingren Zhike mixture.