Abstract: OBJECTIVE To establish the HPLC fingerprint and determine the contents of seven compounds in Niuhuang Qinggan capsules simultaneously. METHODS The separation was carried out on an Agilent SB-C₁₈ column(250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile(A)-0.1% acetic acid solution(B) with gradient elution, the detection wavelength was 210 nm, and column temperature was 30℃. "Similarity evaluation system for chromatographic fingerprint of TCM (2004A)" was used to conduct, and the seven markers were quantified. RESULTS The common mode of the fingerprint showed 22 common peaks, 7 of which were identified by comparison with the reference substances. They were chlorogenic acid, caffeic acid, luteolin-7-o-glucoside, baicalin, baicalein, wogonoside and wogonin. The similar degrees of 12 batches of samples were > 0.90, and the average recoveries varied from 99.1%-104.8% with RSDs of 1.30%-1.88%. CONCLUSION The established method can be used for the quality control of Niuhuang Qinggan capsules.