Abstract: OBJECTIVE To investigate the effect of Kangfuxin suppository on the contents of malondialdehyde(MDA) and superoxide dismutase(SOD) in serum and prostaglandin E2(PGE2) in vaginal tissue of rats with chronic vaginitis. METHODS The rat vaginitis model was established by cross infection of Escherichia coli, Candida albicans, Staphylococcus aureus mixture and 8% phenolic gel. Transvaginal administration of blank suppository, metronidazole suppository (37.84 mg·kg^-1), high dose (80 mg·kg^-1) and low dose (40 mg·kg^-1) of Kangfuxin suppository. The contents of MDA, SOD in serum and PGE2 in vaginal tissue of rats were detected by ELISA. The degree of vaginitis was diagnosed by histopathological score. RESULTS Compared with normal control group, the level of MDA in serum and PGE2 content in vaginal homogenate of rats in model control group increased significantly(P<0.01), SOD activity decreased significantly(P<0.01). The histopathological score of rats' vagina was significantly increased(P<0.01), indicating that the rat vaginitis model was successfully established. Compared with the model control group, the serum SOD activity in the metronidazole suppository group, Kangfuxin suppository high and low groups increased significantly(P<0.01), and the MDA and PGE2 contents decreased significantly(P< 0.01). The histopathological score of rats' vagina was significantly decreased(P<0.01). CONCLUSION Kangfuxin suppository can effectively treat chronic vaginitis of rats, which may be related to reducing the expression of MDA in rats' serum, increasing the activity of SOD in vaginal tissue and changing the degree of pathological changes in vaginal tissue.