Abstract: OBJECTIVE To investigate the control and safety of human prothrombin complex in perioperative blood loss during multi-segment lumbar internal fixation. METHODS One hundred and nine patients with multi-segment lumbar internal fixation were randomly divided into two groups. The study group(57 patients) received intravenous infusion of human prothrombin complex during the operation, and the control group(52 patients) received the same physiological dose brine. Perioperative blood loss, transfusion rate, hemoglobin(Hb), D-dimer, fibrinogen(FIB), prothrombin time(PT), activated partial prothrombin time(APTT) and the complications were compared between the two groups. RESULTS The blood loss, postoperative recessive red blood cell loss, incision drainage volume after 12, 48 h of surgery, total blood loss, intraoperative blood transfusion rate, postoperative blood transfusion rate and total blood transfusion rate were significant lower in the study group than in the control group(P<0.05 or P<0.01). The D-dimer and FIB levels in the control group were gradually decreased(P<0.05), and the levels of PT and APTT were gradually increased (P<0.05), while there was no significant change in the study group. The perioperative Hb level of the study group was higher than that of the control group (P<0.05). There was no significant difference in the complication rate between the study group and the control group. CONCLUSION The human prothrombin complex can effectively control the perioperative hemorrhage of multi-segment lumbar internal fixation, reduce the blood transfusion rate, and does not increase the risk of venous thrombosis, and is safe and reliable.