Abstract: OBJECTIVE To establish a specific method for identification of trionycis-shell glue by finding the marker peptide different from glue of tortoise shell, deerhorn glue, donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, Apalone ferox glue and Palea steindachneri glue. METHODS Samples were extracted with water, trypsin was used for enzymolysis. UPLC-Q-TOF/MS and data processing software were used to analyze the enzymolyzed samples to find the marker peptides of trionycis-Shell glue. Specific method was established by Multi-reaction detection of UPLC-Q-TOF/MS. RESULTS The marker peptide of trionycis-shell gluem/z 784.90(n=2) was found. This polypeptide sequence was GETGPVGVTGSVGPAGAR, one of fragmentation of collagen α-2(I) chainTrionyx sinensis. Used this characteristic ions, m/z 784.90(n=2)→872.45, 1 028.55, established an analysis method for identification of trionycis-shell glue. CONCLUSION The established present method was specific, precise and reliable. The research provided a possible approach for trionycis-shell glue.