Abstract: OBJECTIVE To establish quantitative analysis of multi-components to a single-marker(QAMS) for determining contents of six ingredients from Glycyrrhizae Radix et Rhizoma and to verify the accuracy and feasibility of the method. METHODS QAMS was established and validated for Glycyrrhizae Radix et Rhizoma. Liquiritin apioside, isoliquiritin apioside, liquiritiyenin, isoliquiritiyenin and glycyrrhiic acid were selected an analytes while liquiritin was chosen as internal reference substance to evalyaet the quality. The relative correction factor(RCF) of liquiritin to the other five active ingredients were calculated. The method was evaluated by the comparison of quantity between external standard method and QAMS method. RESULTS The contents of six active ingredients in 16 batches of Glycyrrhizae Radix et Rhizoma determined by QAMS method had no significantly different with external standard method. CONCLUSION The determination of 6 components in Glycyrrhizae Radix et Rhizoma by QAMS is accurate and feasible, which can be used for the quality control of Glycyrrhizae Radix et Rhizoma.