Abstract: OBJECTIVE To investigate the relationship between the recovery of geniposide on self-made linear microdialysis probes and flow rates, drug concentrations, and dialysis membrane lengths. METHODS The concentrations of microdialysis samples were determined by HPLC. The effects of different perfusion speed, different drug concentration and different dialysis membrane length on the in vitro and in vitro forward, reverse recovery and in vivo reverse recovery were investigated. RESULTS The self-made probe was stable. The microdialysis recovery of geniposide was independent of geniposide concentrations, inversely proportional to the flow rates and increased as the length of the dialysis membrane lengths increased. The recovery of positive dialysis and retrodialysis in vitro was significant different(P<0.01). The recovery of in vivo retrodialysis was lower than that of in vitro retrodialysis but was similar to that of in vitro positive dialysis. The recovery of new probe was higer than that used one time in vivo(P<0.05). CONCLUSION It is advisable to use the recovery of in vivo retrodialysis or in vitro positive dialysis method for geniposide skin pharmacokinetic study.