Abstract: OBJECTIVE To explore the underlying mechanism of Baoshenpaidu decoctio(BSD) in sepsis and acute kidney injury (AKI) treatment with network pharmacology analysis. METHODS TCM related databases like TCMSP, TCMID, TCM-Mesh were applied companion with ADME and literature references to screen active ingredients of BSD. Targets of BSD for sepsis and AKI treatment were selected from the overlapping of the predicted targets for ingredients and the obtained targets of the disease. The protein-protein interaction (PPI) network of the targets was constructed by STRING and analyzed by Cytoscape. Clue GO tool in Cytoscape were used to analyze the gene function and KEGG pathway of the targets, and to construct the main drug ingredients-target-pathway network of BSD. RESULTS The 56 main drug ingredients of BSD and 81 related targets for disease treatment were screened from the database analysis. Six hub targets ALB, TP53, VEGFA, PTGS2, EGFR, CASP3 were selected from the PPI network. Gene functional and KEGG pathway analysis showed that these targets were related with virus infection, inflammatory response, reactive oxygen species metabolic, apoptotic process, vascular process in circulatory system, vascular endothelium cells, neutrophil mediated killing of bacterium. Main drug ingredients-targets-pathways network of BSD revealed a multiple-ingredients, multiple-targets, multiple pathways synergistic mechanism. CONCLUSION BSD may play a therapeutic role in sepsis and AKI by multiple-ingredients, multiple-targets, multiple pathways, which might be through the regulation of virus infection, inflammatory response, vascular endothelium cells and other pathways.