Abstract: OBJECTIVE To analyze and compare volatile oils from celery seed by different extraction methods and their effects on proliferation of HSC-LX2 induced by TGF-β1. METHODS The volatile oils were extracted by Soxhlet extraction method and steam distillation, their volatile oils were identified by gas chromatography-mass spectrometry(GC-MS), and experimental data were comprehesively evaluated by using principal component analysis(PCA). CCK-8 assay was used to detect the effect of volatile oil from celery seed by different extraction methods on proliferation of HSC-LX2 induced by TGF-β1. Determination of laminin, hyaluronic acid and type Ⅲ procollagen peptide in hepatic fibrosis indicators of HSC-LX2 cells by enzyme-linked immunosorbent assay. RESULTS Thirty-six kinds of volatile oils extracted by Soxhlet extraction method were separated and identified, while there were twenty-six kinds of volatile oils extracted by stream distillation. There were thirteen volatile oils in common. CCK-8 method showed that the volatile oil of celery seed extracted by Soxhlet extraction method had a stronger inhibitory effect on proliferation of HSC-LX2 induced by TGF-b1 than that by steam distillation. The hepatic fibrosis indicators in the cell supernatant of high-dose volatile oil from celery seed extracted by steam distillation and Soxhlet extraction method was significantly reduced compared with the model group (P<0.01), while the hepatic fibrosis index of Soxhlet extraction method group was closer to that of the negative control group. PCA results showed that the first one was selected as the main component, containing 95.277% information of the original index. 2-butyny-1,4-diol diacetate, 5-methyl-1-hexanol, 2,5-dihydro-1-nitroso pyrrole, 2-hydroxy-trans-cyclohexanonitrile and 2-methyl-1,6-heptadiene were contributed to anti-hepatic fibrosis a lot. CONCLUSION The volatile oil extracted from celery seed by the two methods has great differences in type and content, and the anti-hepatic fibrosis effect is also different in vitro, which lays a foundation for further study on the volatile oil of celery seed and its anti-hepatic fibrosis mechanism.