Abstract: OBJECTIVE To investigate the stability of etoposide, epirubicin and cyclophosphamide injection and provide a basis on clinical usage. METHODS Drug contents, pH values and insoluble particles were determined in three storage situation as 25℃, 25℃ in the dark and 4℃ in the dark, respectively for 24 h. RESULTS The contents, pH values and insoluble particles were stable in three situations. CONCLUSION The compatibility of etoposide, epirubicin and cyclophosphamide has good stability during 24 h, which provide a basis for clinical usage.