Abstract: OBJECTIVE A CO₂-Supercritical Fluid Chromatography(CO₂-SFC) method was established for the simultaneous determination of furanodiene, curdione and germacrone in the Zedoary Turmeric Oil. METHODS A UPC² HSS C₁₈ SB analytical column(3.0 mm×150 mm, 1.8 μm) was adopted and the mobile phase consisted of CO₂-acetonitrile with linear gradient elution(0-8 min, 99.5%→84% of CO₂) at the flow rate of 1 mL·min^-1. The detection wavelength was 216 nm, the column temperature was maintained at 55℃, and the back pressure was was kept at 2 000 psi. RESULTS The linear range of furanodiene was 2.67-1 337.26 μg·mL^-1 (r=1.000), and the average recovery(n=6) was 97.94% with RSD of 1.50%. The linear range of curdione was 2.77-1 386.00 μg·mL^-1 (r=1.000), and the average recovery(n=6) was 96.07% with RSD of 1.68%. The linear range of germacrone was 6.99-3 493.00 μg·mL^-1 (r=1.000), and the average recovery(n=6) was 99.33% with RSD of 1.88%. CONCLUSION The established method is rapid, accurate, and it can protect environment, which provides a new method for the quality control of furanodiene, curdione and germacrone in the Zedoary Turmeric Oil.