Abstract: OBJECTIVE To analysis the reason of the abnormal content transfer rate in standard decoction of Ligustri Lucidi Fructus. METHODS Through visual observation and microscopic identification, the differences between different producing areas and different batches of Ligustri Lucidi Fructus in character and microscopic identification were compared. HPLC was used to compare the fingerprints of 11 batches of powder of Ligustri Lucidi Fructus and 11 batches shell of Ligustri Lucidi Fructus. At the same time, the fingerprints of shell and powder of the same batch of Ligustri Lucidi Fructus were compared. RESULTS There were differences in the appearance and shape of different batches of Ligustri Lucidi Fructus from different producing areas. It was consistent with pharmacopoeia description in microscopic identification. The similarity between 11 batches of Ligustri Lucidi Fructus powder and 11 batches of shell fingerprint was >0.88. The content of specnuezhenide in the shell was 1.15 to 3.5 times that of the powder in the same batch. CONCLUSION There is a significant difference in the content of specnuezhenide in the shell and powder of Ligustri Lucidi Fructus. The degree of wall breaking is different in the study of standard decoction. Therefore, the transfer rate of content of specnuezhenide in standard decoction is abnormal. It is suggested that the ratio of shell to powder or the degree of tamping should be regulated in the production to ensure the stability of inter-batch content.