Abstract: OBJECTIVE To determine 7 components(neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, luteoloside, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C) in Lonicerae japonicae flos standard decoction simultaneously by quantitative analysis of multi-components by single-marker(QAMS). METHODS HPLC analysis was performed on an Agilent Eclipse XDB-C₁₈ column(250 mm×4.6 mm, 5 μm) with a mobile phase consisted of acetonitrile-0.1% acetic acid solution in gradient elution manner at a flow rate of 1.0 mL·min^-1. The column temperature was maintained at 35℃, the detection wavelength was set at 327 nm. Chlorogenic acid was used as the internal reference substance, the relative calibration factors(RCFs) of other 6 components were calculated respectively. The validity of the QAMS method was evaluated to realize QAMS. At the same time, the durability of each RCF was investigated. RESULTS No significant differences were found among the quantitative results of 7 components in Lonicerae japonicae flos standard decoction determined by QAMS and external standard method. CONCLUSION The QAMS method is simple, accurate and reproducible, and can be used to evaluate the quality of Lonicerae japonicae flos standard decoction.