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    清火退热口服液的特征图谱及5种成分的含量测定

    Study on HPLC Characteristic Spectrum of Qinghuo Tuire Liquid and Determination of Five Active Components

      摘要
    • 摘要: 目的 采用HPLC建立清火退热口服液的特征图谱，并同时测定5种成分的含量。方法 以绿原酸、葛根素、连翘酯苷B、连翘酯苷A和黄芩苷为对照品；采用CAPCELL PAKC18 MGⅡ S5（250 mm×4.6 mm，5 μm）为色谱柱，流动相为甲醇-0.5%磷酸水溶液，线性梯度洗脱，流速1.0 mL·min-1，检测波长305 nm，柱温30℃。采用中药色谱指纹图谱相似度评价系统对结果进行分析。结果 绿原酸、葛根素、连翘酯苷B、连翘酯苷A和黄芩苷分别在3.06~61.16，6.51~130.22，4.68~93.55，3.43~68.56，14.61~292.28 μg·mL-1内呈良好的线性关系，相关系数均≥0.999 9；平均加样回收率分别为98.81%，98.32%，95.99%，98.59%，97.26%。结论 所建立的特征图谱相关性强，可结合5种成分含量测定全面控制清火退热口服液的质量。

       

      Abstract: OBJECTIVE To establish an HPLC characteristic spectrum of Qinghuo Tuire liquid and simultaneous determination of five active components. METHODS With chlorogenic acid, puerarin, forsythoside B, forsythoside A and baicalin as reference substance. HPLC was carried on a CAPCELL PAKC18 MGⅡ S5 column(4.6 mm×250 mm, 5 μm) with the column temperature of 30℃; the mobile phase was methanol-0.5% phosphoric acid solution(gradient elution) at the flow rate of 1.0 mL·min-1; the detection wavelength was 305 nm. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate the similar degree. RESULTS The linear ranges of chlorogenic acid, puerarin, forsythoside B, forsythoside A and baicalin were 3.06-61.16, 6.51-130.22, 4.68-93.55, 3.43-68.56, 14.61-292.28 μg.mL-1 respectively (r ≥ 0.999 9), and the average recoveries of 5 components were 98.81%, 98.32%, 95.99%, 98.59%, 97.26% respectively. CONCLUSION With a good correlation, the characteristic spectrum can be combined with the determination of 5 components to control the quality of Qinghuo Tuire liquid comprehensively.

       

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