Abstract: OBJECTIVE To establish the HPLC fingerprint of Fufang Ganmaoling fluid extract and analyze its chemical components for its quality control and medicinal substance study. METHODS Kromasil C₁₈ column(4.6 mm×250 mm, 5 μm) was used with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution, and the flow rate was 1.0 mL·min^-1. The detection wavelength was 290 nm, the column temperature was set at 30℃. Ten batches of Fufang ganmaoling fluid extract were detected, their HPLC fingerprint was established by using Similarity Evaluation System for Chromatographic Fingerprint, and the chemical components in common peaks were identified by reference standard and HPLC-Q-TOF-MS/MS. RESULTS Fingerprints of Fufang Ganmaoling fluid extract were established and 12 common peaks were identified. The peaks were well separated, and the relative retention time of common peaks in each batch was less than 3.0% respectively; the similarity was >0.9 among different samples and 12 chemical components were identified as follows:quinic acid, protocatechuic acid, neochlorogenic acid, swertiajaposide F, chlorogenic acid, cryptochlorogenic acid, caffeic acid, dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and buddleoside. CONCLUSION The method can be used for the quality control of Fufang Ganmaoling fluid extract with good precision, reproducibility and stability, providing basis for further studies on therapeutic efficacy of Fufang Ganmaoling fluid extract.