Abstract: OBJECTIVE To investigate the optimum degreasing process of active composition obtained by bionic enzymatic hydrolysis of Gecko.METHODS The bionic enzymatic hydrolysis solution of Gecko was degreased by solid paraffin method. The yield of extract and the content of active components(total small peptide of Gecko) were used as the evaluation indexes. The optimal degreasing parameters were selected by single factor test and orthogonal test respectively with the concentration of the hydrolyzed solution, the amount of solid paraffin, the times of degreasing and refrigeration time and temperature as the influencing factors. RESULTS The optimized degreasing process parameters were as follows:taking the solution of bionic enzymatic hydrolysis of taking Gecko biomimetic enzymatic hydrolysate, adding 40% solid paraffin while hot, heating and stirring to melt completely, keeping micro-boiling for 5 min, cooling quietly to room temperature, refrigerating(2-4℃) for 6 h, filtering, separating paraffin layer, adding appropriate amount of water to wash twice, and merging filtrate. CONCLUSION The selected degreasing process is stable and feasible, with good degreasing effect and less loss of effective components, which is helpful to the subsequent preparation forming and can provide reference for the degreasing process similar to animal medicine preparations.