Abstract: OBJECTIVE To explore the protective effect of Qidan Fugan granules on lung tissue of mice infected with respiratory virus and its effect on TLRs signaling pathway. METHODS One hundred and twenty mice were randomly divided to control group, model group, positive drug group(ribavirin 100 mg·kg^-1), low, medium and high dose groups(Qidan Fugan granules 1, 2, 4 g·kg^-1), 20 mice in each group, 12 of them were used to observe deaths. Except the control group, the other groups of mice established a model of influenza virus infection by nasal feeding PR8. The morphology of lung tissue was detected by HE staining. The concentration of TNF-α, IL-1β, IL-6 in serum and bronchoalveolar lavage fluid(BALF) were detected by ELISA. Western blot and RT-qPCR were used to detect TLR4/7, MyD88, NF-κB protein and mRNA levels. RESULTS On the 7th day, all mice in the model group died, with a mortality rate of 100.0%. On the 14th day after the establishment of the model, 3(25.0%) died in the positive drug group, 9(75.0%), 6(50.0%) and 5(41.7%) died respectively in the low, medium and high dose groups. On the 5th day, in the model group, positive drug group, low, medium and high dose groups the levels of TNF-α, IL-6, IL-1β in serum and BALF, and the expression of TLR4/7, MyD88, NF-κB protein, the levels of mRNA in lung tissue were significantly higher than the control group(P<0.05). The low, medium and high dose group were significantly lower than the model group(P<0.05), and the middle and high dose groups were significantly lower than the low dose group(P<0.05). There was no significant difference between the medium and high dose groups. CONCLUSION Qidan Fugan granules can alleviate lung injury caused by influenza virus. It may be to inhibit the release of inflammatory factors by inhibiting the expression of TLR4/7-MyD88-NF-κB pathway, thereby reducing lung injury.