Abstract: OBJECTIVE To investigate antitumor effect of zedoray turmeric oil(ZTO) on nude mice bearing ovarian cancer and explore its synergistic effect with cisplatin. METHODS After the establishment of HO-8910PM tumor-bearing mice model, the mice were randomly divided into four groups:model control group(normal saline); ZTO group (300 mg·kg^-1); cisplatin group (2 mg·kg^-1); combination group(300 mg·kg^-1 ZTO+2 mg·kg^-1 cisplatin). The nude mice were sacrificed after three weeks of treatment, tumor volume in each group were detected and tumor inhibition rate were calculated. The expression of NM23, TNF-α, VEGF, NF-KBP65, PCNA in tumor tissues were determined by immunohistochemical. ELISA analysis was performed to evaluate the serum level of IL-2 and IFN-γon in nude mice. RESULTS Compared with model group, the tumor volume were significantly decreased in ZTO, cisplatin and combination group(P<0.05 or P<0.01). And the tumor inhibition rates were 20.55%, 52.52%, 55.06%, respectively. Immunohistochemical results showed that the expression of NM23, TNF-α, VEGF, NF-KBP65, PCNA could be significantly decreased with the administration of ZTO, cisplatin, ZTO and cisplatin combined (P<0.05 or P<0.01), and the tendency from low to high were ZTO, cisplatin, ZTO and cisplatin combined in turn. The levels of IL-2 and IFN-γ were also significantly increased with treatment of cisplatin as well as ZTO and cisplatin combined (P<0.01), and the tendency from low to high was the same to above. CONCLUSION ZTO has the anti-tumor effect on ovarian cancer, the underlying mechanism maybe related with regulation of the expression of tumor growth cytokine NM23, TNF-α, VEGF, NF-KBP65, PCNA and immune-related factors IL-2, IFN-γ. And ZTO combined with cisplatin have potential synergistic attenuated the antitumor effect in nude mice of ovarian cancer.