Abstract: With cancer chemotherapy, radiation therapy, and organ transplants generally carried out, and broad-spectrum antibiotics widely used in clinic, incidence of Candida albicans, Cryptococcus neoformans and other invasive fungal infections incidence has been rising sharply, which is a serious threaten to human health. At present, antifungal agents can be divided into seven categories according to their action targets. Nevertheless, the clinical application of antifungal agents were limited for their serious resistance, narrow antifungal spectrum and adverse effects. Thus, the development of new safe and effective antifungal agents has become a difficult problem to be solved. This paper reviewed the classification, resistance mechanisms, and the new development of antifungal agents, in order to provide reference for the future research direction.