Abstract: OBJECTIVE To establish a simple and rapid dispersive liquid-liquid extraction method (DLLE) to remove the stromal interference components in traditional Chinese medicine cosmetics, so as to isolate the target active components, and to determine the isolated content. METHODS Sample was dissolved in methanol, then the sample solution was filtered and the filtrate pH was adjusted to acidic. The 1.0 mL filtrate was measured precisely into a centrifuge tube, then 3 mL chloroform which was saturated with water and 0.5 mL water were added successively. Subsequently, the mixture was vortexed for 2 min to form a homogeneous emulsion. Then the cloudy solution was centrifuged for 5 min, and the supernatant was collected. As a result, the removal of impurity and the seperation of target content were both accomplished. RESULTS By using the DLLE method for pretreatment of cosmetics sample, targeted active ingredient was isolated, and the recovery of method was between 97.3% to 98.0%, and the methed has good precision and repeatability. The saponins values that was measured in three batches of dermatological Chinese medicine cosmetics were 0.236, 0.210, 0.222 mg·g^-1, respectively. CONCLUSION The DLLE method developed in this paper is simple, and can effectively eliminate the interference and isolate targeted ingredient. In addition the methed has high recovery rate,low organic solvent consumption and thus provides a good analysis method for the determination of saponins in Chinese medicine cosmetics.