Abstract: OBJECTIVE To establish an HPLC method for simultaneous determination of verbascoside, paeoniflorin and baicalin in Kuntai capsules, provide reference for the improvement of the existing quality standards. METHODS Using Phenomsil C₁₈(4.6 mm×250 mm, 5 μm) chromatography column; mobile phase was acetonitrile(A)-0.1% acetic(B) with gradient elution; flow rate was 1.0 mL·min^-1; column temperature was 35℃; the detection wavelength of verbascoside, paeoniflorin and baicalin was 334, 230, 280 nm, respectively. RESULTS Verbascoside had good linear relationship in 3.27-32.68 μg·mL^-1, the average recovery was 98.4%, RSD was 0.68%(n=6). Paeoniflorin had good linear relationship in 17.65-176.51 μg·mL^-1, the average recovery was 99.2%, RSD was 1.08%(n=6). Baicalin had good linear relationship in 48.78-487.77 μg·mL^-1, the average recovery was 97.7%, RSD was 0.87%(n=6). CONCLUSION The method is simple, shortcut, accurate, repeatable and can be used for the quality control of Kuntai capsules.