Abstract: OBJECTIVE To develop a new polymerase chain reaction(PCR) technology for identifying the authenticity of Zheguijiathe dorsal and abdominal carapaces of Clemmys mutica (Cantor). METHODS Extract genomic DNA from Zheguijia and its adulterants was extracted. A series of sequences from mtDNA of Zheguijia and its adulterants were downloaded from the GenBank for analysis and comparison. And the Oligo software was used to design a set of primers, according to the specific sites on Cytb genes of Zheguijia. PCR technology was performed out to amplify the specific fragment of Zheguijia and its adulterants. RESULTS The designed specific primers were only used to amplify the genuine products of Zheguijia and the amplified fragments were 357 bp, which could effectively distinguished Zheguijia and its adulterants. CONCLUSION The PCR assay proposed in this study can be used for the quality control of Zheguijia, which is specificity, sensitivity and applicability.