Abstract: OBJECTIVE To investigate the purification technology conditions of total flavones from Dendrobium officinale leaves by macroporous resin. METHODS Taking adsorption and desorption rate as index ingredients, the optimum macroporous resin was screened by static adsorption-elution test; single factor tests were adopted to investigate sample concentration, sample volume, eluting solvent and solvent volume using total flavones contents as an index to optimize conditions for purification of total flavones from Dendrobium officinale by microporous resins. RESULTS ADS-17 macroporous resin was the optimal choice in purification of total flavones from Dendrobium officinale leaves, the ideal technology conditions were as followed:total flavones concentration of sample was 0.7 mg·mL^-1, sample volume was 30 mL, eluting solvent was 50% aqueous ethanol, and eluant volume was 7 BV. the purity of total flavones was (18.07±0.42)%, the yield rate was (39.91±0.30)%. CONCLUSION ADS-17 microporous adsorption resin can effectively separate and purify total flavones from Dendrobium officinale leaves under the determined process condition and the content of flavones was raised by nearly 17 times than before.