Abstract: OBJECTIVE To establish a new method for quantitative analysis on multi-components with a single marker (QAMS) for simultaneous determination of 7 main constituents including alkaloid and flavone in Compound Kushen Injection(CKI) and to validate its feasibility and technical adaptability in analysis of CKI. METHODS Oxymatrine was used as the internal reference substances and the relative correlation factors(f) of oxysophocarpine, sophoridine, N-methylcytisine, matrine, sophocarpine, trifolirhizin were calculated. RESULTS The relative correlation factors (f) were 0.789, 1.872, 0.408, 1.533, 0.953, 0.409 for oxysophocarpine, sophoridine, N-methylcytisine, matrine, sophocarpine and trifolirhizin respectively. The determination results were compared with those obtained by the external standard method. There was no significant difference in assay results between the QAMS method and the external standard method. CONCLUSION The QAMS method was reliable and can be used for quality control of CKI.